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KMID : 0624620080410030259
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BMB Reports
2008 Volume.41 No. 3 p.259 ~ p.265
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Molecular cloning of peroxidase cDNAs from dehydration-treated fibrous roots of sweetpotato and their differential expression in response to stress
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Kim Yun-Hee
Yang Kyoung-Sil
Kim Cha-Young
Ryu Sun-Hwa
Song Wan-Keun
Kwon Suk-Yoon
Lee Haeng-Soon
Bang Jae-Wook
Kwak Sang-Soo
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Abstract
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Three peroxidase (POD) cDNAs were isolated from dehydration-treated fibrous roots of sweetpotato (Ipomoea batatas) plant via the screening of a cDNA library, and their expressions were assessed to characterize functions of each POD in relation to environmental stress. Three PODs were divided into two groups, designated the basic PODs (swpb4, swpb5) and the anionic PODs (swpa7), on the basis of the pI values of mature proteins. Fluorescence microscope analysis indicated that three PODs are secreted into the extracellular space. RTPCR analysis revealed that POD genes have diverse expression patterns in a variety of plant tissues. Swpb4 was abundantly expressed in stem tissues, whereas the expression levels of swpb5 and swpa7 transcripts were high in fibrous and thick pigmented roots. Swpb4 and swpa7 showed abundant expression levels in suspension cultured cells. Three POD genes responded differently in the leaf and fibrous roots in response to a variety of stresses including dehydration, temperature stress, stress-associated chemicals, and pathogenic bacteria.[BMB reports 2008; 41(3): 259-265]
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KEYWORD
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Dehydration, Environmental stress, Fibrous root, Peroxidase, Sweetpotato (Ipomoea batatas)
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